胰淀素对小鼠脑内类固醇合成急性调节蛋白表达的影响

doi:10.11843/j.issn.0366-6964.2019.11.022

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (11): 2365-2370.doi: 10.11843/j.issn.0366-6964.2019.11.022

• 研究简报 • 上一篇

胰淀素对小鼠脑内类固醇合成急性调节蛋白表达的影响

曹晓娟¹, 李强², 范奎奎², 潘登², 刘昊东¹, 王昆³, 海日汗¹, 杜晨光^1,2*

1. 内蒙古农业大学职业技术学院, 包头 014109;
2. 内蒙古农业大学兽医学院, 呼和浩特 010018;
3. 河北省农林科学院粮油作物研究所, 石家庄 050000

收稿日期:2019-05-29 出版日期:2019-11-23 发布日期:2019-11-23
通讯作者: 杜晨光,主要从事动物组织胚胎与发育生物学研究,E-mail:dcglxj@163.com
作者简介:曹晓娟(1979-),女,山西太谷人,硕士,主要从事动物营养与饲料研究,E-mail:zykyk@163.com
基金资助:
国家自然科学基金（31660701）；内蒙古自然科学基金（2018MS03046）；河北省现代农业产业技术体系肉牛产业创新团队建设（HBCT20181302）

Effect of Amylin on the Expression of Steroidogenic Acute Regulatory Protein in Mouse Brain

CAO Xiaojuan¹, LI Qiang², FAN Kuikui², PAN Deng², LIU Haodong¹, WANG Kun³, HAI Rihan¹, DU Chenguang^1,2*

1. Vocational and Technical College, Inner Mongolia Agricultural University, Baotou 014109, China;
2. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
3. Institute of Grain and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050000, China

Received:2019-05-29 Online:2019-11-23 Published:2019-11-23

摘要/Abstract

摘要： 本试验旨在定位类固醇激素合成急性调节蛋白（StAR）在小鼠脑内的分布，并对胰淀素（amylin）是否影响小鼠脑内StAR的表达进行初步研究。购买C57BL/6品系小鼠，标准环境下饲养一个月后，将获得的子一代饲养7周，选取9只体重及健康状况相近的雄性小鼠为实验动物，随机分为3组：生理盐水对照组、amylin注射组和amylin+AC187（amylin特异性抑制剂）注射组，按体重（100 μg·kg^-1）连续注射3 h后，剖取脑组织，采用免疫荧光染色技术研究StAR在脑内的分布规律，同时采用Western blot技术比较不同组中StAR的表达变化规律。试验结果表明，StAR免疫阳性信号主要表达在不定带（ZI），零星分布于蓝斑核（LC）、下丘脑腹内侧核（VMH）、中缝背核（DR）。与对照组相比，腹腔注射amylin（100 μg·kg^-1）极显著降低ZI内StAR表达量（P<0.01）；联合抑制剂处理组amylin+AC187能极显著抑制amylin对ZI内StAR表达的影响（P<0.01）。结果表明，腹腔注射amylin可显著抑制ZI内StAR的表达，由于StAR是调节类固醇激素的限速酶，推测amylin除可调节进食及能量代谢外，还可通过调节StAR的表达影响中枢神经系统内类固醇激素的合成速率。

Abstract: This study aims to localize the distribution of steroidogenic acute regulatory (StAR) protein in the brain of small mice, and to preliminarily investigate whether amylin affects the expression of StAR in the brain of small mice. C57BL/6 strain mice were purchased and fed under standard environment for one month. The offspring were fed for seven weeks. Nine male mice with similar weight and health status were selected as experimental animals, they were randomly divided into 3 groups:normal saline control group, amylin injection group and amylin + AC187 (amylin specific inhibitor) injection group. After 3 hours' continuous injection according to body weight (100 μg·kg^-1), brain tissue was dissected. The distribution of StAR in brain was studied by immunofluorescence staining, and the expression of StAR in different groups was compared by Western blot. The results showed that StAR immunopositive signals were mainly expressed in zone incerta (ZI), sporadically distributed in locus coeruleus (LC), ventromedial hypothalamic nucleus (VMH) and dorsal raphe nucleus (DR). Compared with the control group, intraperitoneal injection of amylin significantly reduced the expression of StAR in ZI (P<0.01); and amylin +AC187 significantly inhibited the effect of amylin on StAR expression in ZI (P<0.01). The results showed that intraperitoneal injection of amylin could significantly inhibit the expression of StAR in ZI. StAR is a rate-limiting enzyme that regulating steroid hormones, we speculate that amylin can not only regulate food intake and energy metabolism, but also affect the synthesis rate of steroids in the central nervous system by regulating the expression of StAR.

中图分类号:

S852.2

曹晓娟, 李强, 范奎奎, 潘登, 刘昊东, 王昆, 海日汗, 杜晨光. 胰淀素对小鼠脑内类固醇合成急性调节蛋白表达的影响[J]. 畜牧兽医学报, 2019, 50(11): 2365-2370.

CAO Xiaojuan, LI Qiang, FAN Kuikui, PAN Deng, LIU Haodong, WANG Kun, HAI Rihan, DU Chenguang. Effect of Amylin on the Expression of Steroidogenic Acute Regulatory Protein in Mouse Brain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2365-2370.

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胰淀素对小鼠脑内类固醇合成急性调节蛋白表达的影响

Effect of Amylin on the Expression of Steroidogenic Acute Regulatory Protein in Mouse Brain

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